Open Access Open Access  Restricted Access Subscription or Fee Access

A combined and intelligent new segmentation method for boar semen based on thresholding and Watershed transform

V. Gonzalez-Castro. Gonzalez-Castro, Enrique Alegre, P. Morala-Arguello, S. A. Suarez

Abstract


This work presents a new method to segment images of alive and dead spermatozoa in positive phase contrast. This method improves previous segmentation methods applying an intelligent threshold combined with watershed segmentation. First, it applies an intelligent thresholding segmentation that changes the value of threshold when the binary image obtained is not fulfill the surface and eccentricity factors. Then, using the same automatic criteria, the bad segmented images are processed by means of the watershed transform. Using this new method a 90.96% of the spermatozoa have been correctly segmented. This approach could be useful to commercial Computer Assisted Semen Analysis systems that need new and more accurate segmentation processes.

Keywords


Segmentation, Thresholding, Watershed, Boar semen

Full Text:

PDF

Refbacks

  • There are currently no refbacks.


Disclaimer/Regarding indexing issue:

We have provided the online access of all issues and papers to the indexing agencies (as given on journal web site). It’s depend on indexing agencies when, how and what manner they can index or not. Hence, we like to inform that on the basis of earlier indexing, we can’t predict the today or future indexing policy of third party (i.e. indexing agencies) as they have right to discontinue any journal at any time without prior information to the journal. So, please neither sends any question nor expects any answer from us on the behalf of third party i.e. indexing agencies.Hence, we will not issue any certificate or letter for indexing issue. Our role is just to provide the online access to them. So we do properly this and one can visit indexing agencies website to get the authentic information.